Oscar Juarez

Education

Ph.D. National University of Mexico
B.S. National University of Mexico

Research Interests

本实验室开展生物医学研究，旨在建立细菌致病性物种的代谢策略和宿主-病原体关系, such as Chlamydia trachomatis世界上最普遍的性传播疾病，以及 Vibrio cholerae这种疾病使发展中国家数百万人患上严重的胃肠道疾病.

我们的研究采用跨学科的方法来研究这些主题, with diverse methodologies, such as molecular and structural biology, protein chemistry and cell biology. 这种策略使我们能够表征酶的行为，并了解分子和亚原子方面, such as electron movement and conformational changes, 把这些概念应用到细胞间的关系中, disease development and drug design.

Publications

Peer-reviewed publications

• Raba DA, Rosas-Lemus M, Menzer WM, Li C, Fang X, Liang P, Tuz K, Minh DDL, Juarez O (2018) Characterization of the Pseudomonas aeruginosa NQR复合物，细菌质子泵与自中毒抗性的作用. J. Biol. Chem. 2018. In press. doi: 10.1074/jbc.RA118.003194
• Veseli IA, Mascarenhas dos Santos AC, Juárez O, Stark BC, Pombert JF (2018) Complete genome sequence of Vitreoscilla sp. 菌株C1，第一个细菌血红蛋白微生物资源的来源. 2018. 7:e00922-18. DOI: 10.1128/MRA.00922-18
• 梁鹏，Rosas-Lemus M, Patel D, Fang X, Tuz K, Juárez O(2017)动态能源依赖 Chlamydia trachomatis 在细胞内生长的不同阶段宿主细胞代谢:钠基能量学在衣原体ATP生成中的可能作用. J. Biol. Chem. 2018 293:510-. DOI: 10.1074/jbc.M117.797209
• Fang X, Liang P, Raba DA, Rosas-Lemus M, Chakravarthy S, Tuz K, Juárez O (2017) Kinetic characterization of Vibrio cholerae ApbE: Substrate specificity and regulatory mechanisms. PLoS One. 2017 Oct 24;12(10):e0186805. DOI: 10.1371/journal.pone.0186805
• Tuz K, Li C, Fang X, Raba DA, Liang P, Minh DD, Juarez O(2017)霍乱弧菌钠依赖性NADH脱氢酶催化泛素结合位点的鉴定:一种新的泛素结合基元. J Biol Chem. 2017 Feb 17;292(7):3039-3048. DOI: 10.1074/jbc.M116.770982
• Tuz K, Mezic KG, Xu T, Barquera B, Juarez O (2016) Vibrio cholerae sodium-dependent NADH dehydrogenase. J Biol Chem. 2015. 290(33):20009-200021. PMID: 26004776, PMCID: PMC4536408
• Hreha TN, Mezic KG, Herce HD, Duffy EB, Bourges A, Pryshchep S, Juárez O, Barquera B (2016) RNF复合物的完整拓扑结构 Vibrio cholerae. Biochemistry. 2015. 54(15):2443-2455. PMID: 25831459
• Shea ME, Mezic KG, Juárez O, Barquera B. (2015)在K+存在下Na+-NQR的突变使Na+易位的电子流不耦合. Biochemistry. 54(2):490-6
• Strickland M, Juárez O, Neehaul Y, Cook DA, Barquera B, Hellwig P. (2014) Na+泵送NADH:泛醌氧化还原酶(Na+-NQR)中泛醌结合引起的构象变化受NqrB亚基的保守甘氨酸140和141的动力学控制. J Biol Chem. 289(34):23723-33
• Reyes-Prieto A, Barquera B, Juárez O. (2014)钠泵送NADH:泛醌氧化还原酶的起源与进化. PLoS One. 9(5):e96696.
• Shea ME, Juárez O, Cho J, Barquera B. (2013)霍乱弧菌钠离子泵送NADH:醌氧化还原酶B亚基中的天冬氨酸397形成钠结合位点的一部分, is involved in cation selectivity, and affects cation-binding site cooperativity. J Biol Chem. 288(43):31241-9.
• Neehaul Y, Juárez O, Barquera B, Hellwig P. (2013)霍乱弧菌钠离子泵送NADH:醌氧化还原酶中涉及离子结合残基NqrB-D397的氧化还原依赖构象变化的红外光谱证据. Biochemistry. 52(18):3085-93
• Juárez O, Barquera B. (2012)钠离子泵送NADH:醌氧化还原酶的电子转移和钠易位机制研究. Biochim Biophys Acta. 1817(10):1823-32
• Tuz K, Hsiao YC, Juárez O, Shi B, Harmon EY, Phelps IG, Lennartz MR, Glass IA, Doherty D, Ferland RJ. (2013) Abelson-helper整合位点1 (AHI1)蛋白的Joubert综合征相关错义突变(V443D)改变了其定位和蛋白-蛋白相互作用. J Biol Chem. 288(19):13676-94.
• Juárez O, Barquera B. (2012)钠离子泵送NADH:醌氧化还原酶的电子转移和钠易位机制研究. Biochim Biophys Acta. 1817(10):1823-32
• Juárez O, Neehaul Y, Turk E, Chaubon N, Hellwig P和Barquera B. (2012) NqrB亚基甘氨酸残基140和141在钠依赖性NADH脱氢酶泛醌结合位点中的作用. J Biol Chem. 287(30):25678-85
• Neehaul Y, Juárez O, Barquera B, Hellwig P. (2012)霍乱弧菌钠离子泵送NADH:醌氧化还原酶电子转移调控的热力学贡献. Biochemistry. 51 (19): 4072–4077
• Juárez O, Shea ME, Makhatadze GI, Barquera B. (2011)霍乱弧菌钠离子泵送NADH:醌氧化还原酶催化和调节阳离子结合位点的作用和特异性. J Biol Chem. 286(30):26383-90.
• De Los Rios Castillo D, Zarco-Zavala M, Olvera-Sanchez S, Pardo JP, Juárez O, Martinez F, Mendoza-Hernandez G, García-Trejo JJ, Flores-Herrera O. (2011)人合胞滋养层线粒体的非典型嵴形态:复合体V的作用. J Biol Chem. 286(27):23911-9.
• Juárez O, Morgan JE, Nilges M, Barquera B. (2010)霍乱弧菌Na+-NQR的能量转导氧化还原步骤. Proc. Natl. Acad. Sci. 107(28):12505-10.
• Juárez O, Athearn K, Gillespie P, Barquera B(2009)参与Na+-NQR钠转运机制的酸残基. Biochemistry. 48(40):9516-24.
• Juárez O, Morgan JE, Barquera B(2009)霍乱弧菌钠离子泵送NADH:醌氧化还原酶的电子传递途径. J. Biol. Chem. 284(13):8963-72.
• Juárez O, Nilges MJ, Gillespie P, Cotton J, Barquera B (2008). 核黄素是霍乱弧菌钠离子泵送NADH:醌氧化还原酶(Na+-NQR)的活性氧化还原辅助因子. J. Biol. Chem. 283(48):33162-7.
• Backiel J, Juárez O, Zagorevski DV, Wang Z, Nilges MJ, Barquera B(2008)霍乱弧菌Rnf复合物中黄素与RnfG和RnfD的共价结合. Biochemistry 47(43):11273-84.
• Guevara-Flores A, Olvera-Sánchez S, Gómez-Concha C, Juárez O, Esparza-Perusquía M, Pardo JP, Mendoza-Hernández G, Martínez F, Flores-Herrera O(2008) 5'-对氟磺酰苯甲酰腺苷抑制带绦虫囊尾蚴被皮表面的外链atp二磷酸水解酶. Mol Biochem Parasitol. 162(2):123-33.
• Juárez O, Guerra G, Velázquez I, Flores-Herrera O, Rivera-Pérez RE, 张建平(2006)小麦黑穗病的生理研究. FEBS J. 273(20):4603-15.
• Juárez O, Guerra G, Martínez F, Pardo JP(2004)马氏菌线粒体呼吸链. Biochim Biophys Acta. 1658(3):244-51.

Book chapters

• Juarez O, Barquera B. (2010) Sodium pumping NADH dehydrogenase. In: Biochemical Communication (Ed. Vazquez-Meza), Mexico City, UNAM, p. 116.
• Juárez O, Pardo JP. Structure and function of the alternative oxidase. In: Advances in Protein Physical Chemistry (Ed. Fernandez-Velasco A), Kerala, Research Signpost, p. 303.
• Rendón JL, Juárez O(2009)谷胱甘肽还原酶的结构、催化和功能研究. In: Advances in Protein Physical Chemistry (Ed. Fernandez-Velasco A), Kerala, Research Signpost, p. 317.

Projects

1. 致病菌的代谢适应及宿主-病原体关系的研究
2. 参与离子稳态和能量产生的酶和蛋白质的结构和功能分析
3. Rational design of drugs against pathogenic bacteria

Expertise

机制酶学，代谢网络表征，和蛋白质化学